A9594

COS 7 cells transfected with pFLAG-CMV2-BAP plasmid stained with with ANTI-FLAG Cy3 Conjugate (A 9594).

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A9594

Sigma

 

Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution

Synonym:Monoclonal ANTI-FLAG® M2 antibody produced in mouse

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Description

ApplicationFor simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
SpecificityBinds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Physical formSolution in phosphate buffered saline plus 1% BSA and preservative
Legal InformationCy3 is a trademark of GE Healthcare
 ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
 FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formpurified immunoglobulin
cloneM2 (monoclonal)
formbuffered aqueous solution
application(s)direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotypeIgG1
shipped indry ice
storage temp.−20°C

Safety

WGK Germany2